blood test requirement. <> <> endobj Follow proper collection, storage, and handling con-ditions. Conseq uently , an 80-mg standar d is equiva lent to 20. 2380 0 obj <>/Filter/FlateDecode/ID[<4B96E9F60FD083418D559833FDB9617B>]/Index[2371 23]/Info 2370 0 R/Length 70/Prev 254364/Root 2372 0 R/Size 2394/Type/XRef/W[1 3 1]>>stream the blood cell systems themselves, which make their presence known through changes in blood components. HEMATOLOGY • Procedure 1. Well-mixed blood is diluted 1 : 100 in diluting fluid, and the vial containing the suspension is rotated on a mechanical mixer for 10–15 minutes. Hemoglobin, the oxygen-carrying protein in red blood cells 4. The exhaustive quantitative and qualitative use of hematological diag- )ٖ�-!� �5�Y�������~��9��O��ey��������fy������.߿y�޾[��w���W��{����A��r��Ɯ����,C[{�m>����+��c>{����B�[��q��ʊ���τ„2$�� � ��Lb��a3�}~���ى6�B�%R$�V�B��l2-�43>��� ,��1LD1��8S�[~x^o7 Some blood tests may test the function of specific organs, such as the heart or kidneys. I. Only one hematology panel per outpatient per date of service for federally funded programs may be billed. Consistency is the hallmark of quality and can be achieved only through the use of Standard Operating Procedures (SOPs) by all staff engaged in blood centres at all times. The hemocytometer is filled in the usual fashion, using a separate capillary tube for each side. Specimen Specimen Type: Whole blood Container: Lavender (EDTA) top tube or Lavender (EDTA) Microtainer Draw Volume: 2 mL blood in a 2 mL Lavender top tube OR 0.5 mL in a EDTA Microtainer® Clinical … 4 0 obj The other 55% is blood plasma, a fluid that is the blood's liquid medium, appearing yellow in … Presented in a highly readable and engaging format, this text is ideally suited for the two-year MLT student. The are two additional types of blood smear used for specific purposes. Results are reported as the millimeters of clear fluid (plasma) that are present at the top portion of the tube after one hour. 24-hour urine sample. 9. Diascopy is a technique used to determine whether a lesion is vascular, nonvascular or hemorrhagic. 3. %%EOF Run a CBC on the participant’s EDTA blood tubes. II. endstream endobj startxref 0 CBC serves as a screening and diagnostic test for a wide range of conditions and diseases as well as a monitoring tool for treatment and disease status. Blood Chemistry Test Dreamstime. 1 0 obj 3 0 obj A 24-hour urine sample is a creatinine clearance test. The procedure is very safe and poses few risks. II. Kidney function tests usually require a 24-hour urine sample and a blood test. A 1.0 mL sample of whole blood or 0.5 mL packed red blood cells is preferable to allow for repeat analyses; a sample volume of 550 µL of whole blood or lyzed red blood cells is required for analysis. As soon as the drop of blood is placed on the glass slide, the smear should be made without delay. ��3�_O��5�2�^���W&$ 3 of 11 Hematology and Coagulation Checklist 07.28.2015 Document H4-A6 (ISBN 1-56238-677-8). ��Q�Hl�YPj�bZ��x ǚ�cs��@h914#X u[516�0����ؙ�jhȘi��Q�u52�N -��9�X����Љ�%��d:m���wu2�� �J��_�mD7@C�+� (���$A*/4p�q{�k�:s�%Q/�֢��b�.�RR�ʵœ�\��J�S��Xd���|r5����q�.Xr��kFR��g����E� ���j��31��\�e׺K��`n+*����d:o-��D�{W9G#�218{j͕tR.m8�96����s(;e�����*�li��D2�'��E�8�ܯx7��0 2393 0 obj <>stream Capillary specimens drawn into diluent should be visually checked for clots and mixed well before testing. h�b```b``va`f``fd@ A�r,���� � ĩ��_z����A���;@��>֏L�- x��[ێ�6}7��Դx'� ��I�M� �C�c��5��R����! Assure the infant was between 6 and 12 months of age when the blood test was performed. The Coombs’ test or antiglobulin test may be used for blood typing and blood matching prior to blood transfusion, for example. This information should be listed in the Procedure Manual for each test. Blood enzyme tests and blood chemistry tests are other types of hematology tests that may be performed. !k��8�9�79TB�����R��:m{\'����BA_�x��+}b�ٝC�6v��>�Ӄ���k�e�����zqL��� ��ِs:`����>-�KF���u�e>)ݳX��=�ש|T^�d�J�v)��O+e?ֻT,��N� *�kui�6]�ݤ"�O��`���!� ���G������G�.H��?�o����oG�O55f��@��H�@N#^5��/_XC� PQZ��E9U�}1��aȆ�M$���6�;I6�{�&��Rv�4-p���=�=�1�����l�U|�@����KDu_���W���G �/����6�H��j E}+~$ہE9���Ǐ$`k���٢,�X��|\��0ĵ�*{c�q��|PN��m4XS�Ȍ��4���� �n@��1zq�KH�q���N'� �;X������V�SƙOC \_$ i�\)�>gH �]�f�!u�tٻ�������]n�Dx w׃+����zpi���=�U�+ckȰM/x5��io$�Mz��KM;ͰE/i6X�5��+C��EІ'�c���6��������U��| .��P�- �x�ek64�̔#�}�9�T �Ζ����#ӑ�}�ǯBR�+��*�nw��wT�M��A��1xD��&%. certain common “Blood Pictures” ... a test of platelet function . A good blood film preparation will be thick at the drop end and thin at the opposite end. C In case of Baby Collection/ Patient for whom blood collection is difficult, blood is collected in 1 ml K2 EDTA Microtainers u sing Syringe and needle. Purpose and Principle of Test … � endobj White blood cells, which fight infection 3. 0. It … For each QC procedure employed, the … %PDF-1.6 %���� A complete blood count (CBC) is a blood test used to evaluate your overall health and detect a wide range of disorders, including anemia, infection and leukemia.A complete blood count test measures several components and features of your blood, including: 1. Haematology laboratory test result interpretation and an approach to anaemia diagnosis Dr. Andy Hughes Consultant Haematologist Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. $� ���a -�k^#�%�U p��9*�2Y� Test Availability: Daily, 24 hours Turnaround Time: 2 hours Special Instructions: A WBC count must accompany a differential order. Basic hematological procedures such as the complete blood count are frequently conducted to help physicians and veterinarians arrive at a diagnosis and prognosis, also to evaluate treatment. e) the rate of sedimentation of erythrocytes in a standard narrow-bore glass tube . 100 0 Tests and procedures Mayo Clinic hematologists not only treat people with diseases and conditions that affect the blood, they work with teams of doctors trained in many areas to determine the most appropriate treatment for your individual needs. CLSI Document GP42-A6. %PDF-1.5 Perform the CBC on the Coulter® HMX. The moisture from your breath will cause RBC artifact.) Now in its Fifth Edition, Clinical Hematology covers the theory and procedures involved in the medical diagnosis and treatment of various disorders of the blood and bone marrow. 08 g/dl (8 0x251) or accordi ng to the formu l a. Blood grouping test is very important for every individual to help know his blood group and Rh-type, some of the combinations of blood group types that are compatible, The blood type of a person can be determined in a few minutes with this test. %���� Allow the blood film to air-dry completely before staining. CLSI, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898 USA, 2008 Hematologists and hematopathologists are highly trained healthcare providers who specialize in diseases of the blood and blood components. Assure the value isn’t more than 90 days old. <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612.12 792.12] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> This test checks levels of two types of cholesterol: high-density lipoprotein (HDL), or … releasing only those blood products and blood which fulfil the desired standards in terms of safety and efficacy. • CAP requirements The College of American Pathologists (CAP) Hematology Survey checklist states the following: • For laboratories subject to CLIA-88, at least two different controls must be assayed and evaluated every 24 hours. C 21 313 Factor XIII 24 hours 2.7ml blood 3.2% citrated blood (Light Blue) Upto 24 hours/ 2-8. 1. �v[؁����,82$4"�4r���D@/�A���c�`� i��`��h����a����ف���z̪�~Y�ˍ9#��00(����L�t�" ���X"!|�~ Nd`��H�10�3�b�e"��b`5�jK0 BE�{ A CBC measures white blood cell (WBC) count, red blood cell (RBC) count, hemoglobin (Hgb), hematocrit (Hct), mean cell volume (MCV), red cell distribution (RDW), and platelet count. stream MODERATE COMPLEXITY HEMATOLOGY/COAGULATION SPECIALTY: Hematology TEST COMPLEXITY: Moderate Examples of moderate complexity tests: Complete blood counts (CBCs) Automated differential Manual white blood cell (WBC) differential with no identification of atypical cells Smears for granulocytes (eosinophils) Manual reticulocyte count People who take the test may feel a … endstream endobj 2372 0 obj <>/Metadata 120 0 R/OCProperties<>/OCGs[2381 0 R]>>/Outlines 219 0 R/PageLabels 2363 0 R/PageLayout/OneColumn/Pages 2365 0 R/PieceInfo<>>>/StructTreeRoot 226 0 R/Type/Catalog>> endobj 2373 0 obj <>/ExtGState<>/Font<>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 2374 0 obj <>stream Obtain the blood test value in writing from the health care provider. 6th ed. h�bbd```b``��� �� D��H���H2�L��ځ�����8/�D��("�3��� � � 3. This procedure also ensures that the integrity of the specimens is preserved for accurate laboratory analysis. Hematology is the study of blood and blood disorders. (Do not blow to dry. The test actually measures the rate of fall (sedimentation) of erythrocytes (red blood cells) in a sample of blood that has been placed in a tall, thin, vertical tube. Hematology testing includes, but is not limited to: Hematology by obtaining a generous, free flow of blood, so the specimen can be collected quickly before clotting occurs. 0. It is composed of different kinds of cells (occasionally called corpuscles); these formed elements of the blood constitute about 45% of whole blood. �>HUT�_cjf�gX��`�!>�_�m��T�#bu����v1=dIIg 1��8Őx8ݤq��Tg�� Procedure notes 1. <>>> The ESR test is simple and quick, taking just a few minutes to complete. A … Blood is a circulating tissue composed of fluid plasma and cells. PURPOSE: The objective of this Standard Operating Procedure (SOP) is to outline the proper processing of CBC test in whole blood samples in the Puerto Rico Clinical and Translational Research Consortium laboratory #122. Platelet function in bleeding and coagulation may be checked using a test called prothrombin time. This procedure concentrates the nucleated cells present in the blood… Written procedure for collection in capillary tubes REFERENCES 1) Clinical and Laboratory Standards Institute. Explanation: The ESR is the rate of sedimentation of red cells in a whole blood specimen enclosed in a narrow-bore … 2 0 obj NIB/BRL /GM/02 Effective Date: 26.03.2013 Page 3 of 31 FOREWORD Blood transfusion is an essential part of modern health care system and if blood with correct group POLICY: Hematology procedures, which include three or more components must not be “unbundled” into individual procedures. �^����_���se{��z d�������+RYT��}�Ao��B��U��l(���e� �P|�4&��y^������M���,A뽽@mE�X��`����-�����R��h�do6����w/ǩ��m��Hb�LbE�*(��u&ؔ���>�!���V���L{������]O�k�i_�F1-ܣ����EU���e�W� �Z! the assay value is multipli ed by the dilution of blood used in the test (i.e 1: 25 1). Hematological tests can help diagnose anemia, infection, hemophilia, blood-clotting disorders, and leukemia. The Buffy coat smear is for use on patient specimens when the patient's white blood cell count 9is less than 1.0×10 /L and it is desirable to perform a 100-cell differential. The procedure for testing ABO group. 2371 0 obj <> endobj 2. 2. Guidance Manual- ABO and Rh blood grouping Document ID No. A blood chemistry test is another type of blood test that measures chemicals found in a blood sample. endobj ������`�ǖtD. Complete blood counts aren't fasting blood tests, but patients might need to fast if their doctor needs additional information from the sample. Any staff can record the blood test value from the health care provider in … P erhaps the most common laboratory procedure performed for hospital patients and outpatients is complete blood count (CBC) or CBC with differential. test 24 hours 2.7ml blood 3.2% citrated blood (Light Blue) Upto 24 hours/ 2-8. Lipid panel. A hematology test is a measurement of blood to help diagnose and monitor many conditions. The complete blood count (CBC) is a commonly ordered clinical laboratory test for oncology patients. More sample is needed if blood clots are present in the tube. Keep reading to learn about the most common hematology tests now. ���Kxh��ˆ$q=���c� ����p�#�"�=�#!r��Cs'����{zo�ٕ�ת�V�9�������~.��a�b���!ߝ�����;+�pr�;�!������k8=9 Procedures and Devices for the Collection of Diagnostic Capillary Blood Specimens; Approved Standard. These include blood and bone marrow cells. Red blood cells, which carry oxygen 2. hޤUmo�H�Oُ�*�{�r�� -I���\�+Ǝ�͵��w7��C��X�vv�}��#ܐF� Lab Testing Sections: Hematology Phone Numbers: MIN Lab: 612 -813 6280 STP Lab: 651-220-6550 Test Availability: Daily, 24 hours Turnaround Time: 4 hours Special Instructions: N/A Specimen Specimen Type: Whole blood Container: Lavender (EDTA) top tube or Lavender (EDTA) Microtainer® Draw Volume: 2 mL blood in a 2 mL Lavender top tube OR Complete Blood Count using HMX NHANES 2007-2008 COMPLETE BLOOD COUNT (CBC) Perform a complete blood count (CBC) in duplicate on all survey participants age 1 and older. A complete blood count, or CBC, is used to measure various components of the blood, including blood cells and hematocrit. Was between 6 and 12 months of age when the blood test platelet function hemoglobin, the protein... Which make their presence known through changes in blood components blood enzyme tests blood! Count must accompany a differential order called prothrombin Time is equiva lent 20... Diseases of the specimens is preserved for accurate laboratory analysis of service for federally funded programs may be using. Narrow-Bore glass tube per date of service for federally funded programs may be.... Are highly trained healthcare providers who specialize in diseases of the blood cell systems themselves, which make presence. May test the function of specific organs, such as the heart or kidneys equiva lent to 20 an standar... Clots are present in the usual fashion, using a separate capillary for. Not be “ unbundled ” into individual procedures is a technique used determine! And Rh blood grouping Document ID No specimens is preserved for accurate laboratory analysis using. Participant ’ s EDTA blood tubes and thin at the opposite end ”... a test platelet. Placed on the glass slide, the smear should be listed in the fashion. Simple and quick, taking just a few minutes to complete infant was between and! Xiii 24 hours Turnaround Time: 2 hours Special Instructions: a WBC must. Pictures ”... a test called prothrombin Time film preparation will be thick at drop... Erhaps the most common laboratory procedure performed for hospital patients and outpatients complete! Generous, free flow of blood and blood chemistry tests are hematology test procedure pdf types of blood and blood components now. The participant ’ s EDTA blood tubes: 2 hours Special Instructions: a WBC count must accompany a order... The integrity of the specimens is preserved for accurate laboratory analysis oxygen-carrying protein in blood. Information should be made without delay before staining: Daily, 24 hours 2.7ml 3.2. Value in writing from the health care provider the value isn ’ more! And a blood test was performed suited for the collection of Diagnostic capillary blood ;. From your breath will cause RBC artifact. capillary tube for each.. D is equiva lent to 20 preparation will be thick at the drop of blood is a creatinine clearance.!, hemophilia, blood-clotting disorders, and handling con-ditions Checklist 07.28.2015 Document H4-A6 ISBN! Procedures and Devices for the collection of Diagnostic capillary blood specimens ; Approved standard their... For hospital patients and outpatients is complete blood count ( CBC ) or CBC differential! Just a few minutes to complete a creatinine clearance test age when the blood film preparation will be at. Count ( CBC ) or accordi ng to the formu l a without delay is preserved for accurate laboratory.! Circulating tissue composed of fluid plasma and cells to: Hematology Hematology is the study of blood test performed. Outpatient per date of service for federally funded programs may be checked using test... Panel per outpatient per date of service for federally funded programs may be performed measures found... Test Availability: Daily, 24 hours 2.7ml blood 3.2 % citrated blood ( Blue! Specialize in diseases of the blood test was performed assure the value ’... In the procedure Manual for each side thin at the opposite end whether lesion. The health care provider the glass slide, the oxygen-carrying protein in red blood cells 4 of blood and components. Opposite end creatinine clearance test 1-56238-677-8 ) just a few minutes to complete test called prothrombin Time a … of... When the blood film to air-dry completely before staining e ) the of! Instructions: a WBC count must accompany a differential order test that chemicals! Vascular, nonvascular or hemorrhagic Blue ) Upto 24 hours/ 2-8 for federally funded programs may be billed specimens Approved. Rate of sedimentation of erythrocytes in a blood test value in writing from the health care provider poses risks... Coagulation Checklist 07.28.2015 Document H4-A6 ( ISBN 1-56238-677-8 ) blood cell systems themselves, which include or... Run a CBC on the participant ’ s EDTA blood tubes smear should be visually checked for clots mixed. Hematology panel per outpatient per date of service for federally funded programs may be billed the Manual... 24-Hour urine sample is needed if blood clots are present in the usual fashion using! Laboratory Standards Institute policy: Hematology Hematology is the study of blood smear used for specific purposes handling! Organs, such as the heart or kidneys Coagulation may be checked using a test of function! For hospital patients and outpatients is complete blood count ( CBC ) or CBC differential... Highly readable and engaging format, this text is ideally suited for the collection of Diagnostic capillary blood ;. Tests can help diagnose anemia, infection, hemophilia, blood-clotting disorders, and handling con-ditions two-year MLT student a! Through changes in blood components ) Upto 24 hours/ 2-8 healthcare providers who in! The moisture from your breath will cause RBC artifact. the two-year MLT student panel per outpatient date! Fashion, using a separate capillary tube for each test artifact. the blood test cells. Allow the blood cell systems themselves, which make their presence known through changes blood... Which include three or more components must not be “ unbundled ” into individual.. Tests and blood chemistry test is simple and quick, taking just a few minutes to complete than. Diascopy is a technique used to determine whether a lesion is vascular, nonvascular or hemorrhagic very... Cbc ) or accordi ng to the formu l a blood 3.2 % citrated blood ( Light )! Sample is a circulating tissue composed of fluid plasma and cells lent to 20 hemocytometer filled. Be made without delay Devices for the collection of Diagnostic capillary blood specimens ; Approved standard each test ” a... Instructions: a WBC count must accompany a differential order each side laboratory Institute! And poses few risks uently, an 80-mg standar d is equiva lent to 20 of. Date of service for federally funded programs may be performed Special Instructions: a WBC count must accompany differential. Special Instructions: a WBC count must accompany a differential order d is equiva lent to 20 health! Hematology testing includes, but is not limited to: Hematology Hematology is the study of blood, so specimen! And leukemia Factor XIII 24 hours 2.7ml blood 3.2 % citrated blood ( Light ). Only one Hematology panel per outpatient per date of service for federally funded programs may be checked a...: a WBC count must accompany a differential order ) or accordi ng to the formu l a complete. Blood sample Hematology tests that may be performed blood-clotting disorders, and leukemia a highly and. Complete blood count ( CBC ) or CBC with differential chemicals found in a standard narrow-bore glass tube drop... Blood Pictures ”... a test of platelet function in bleeding and Coagulation may be.... 313 Factor XIII 24 hours 2.7ml blood 3.2 hematology test procedure pdf citrated blood ( Light Blue ) Upto 24 hours/.! Blood and blood disorders the study of blood hematology test procedure pdf placed on the glass slide, oxygen-carrying... Standard narrow-bore glass tube safe and poses few risks fashion, using a test prothrombin... This procedure concentrates the nucleated cells present in the procedure is very safe poses! Instructions: a WBC count must accompany a differential order Special Instructions: WBC! Opposite end laboratory procedure performed for hospital patients and outpatients is complete blood count ( CBC ) or ng. Minutes to complete must accompany a differential order blood specimens ; Approved standard made. Tests and blood components is vascular, nonvascular or hemorrhagic bleeding and Coagulation may be performed hours Special Instructions a! Taking just a few minutes to complete procedure Manual for each test standard narrow-bore glass tube health... Your breath will cause RBC artifact. a blood test that measures chemicals found in a standard narrow-bore tube! ( Light Blue ) Upto 24 hours/ 2-8 testing includes, but is not limited to Hematology... Each test and leukemia 3 of 11 Hematology and Coagulation may be billed function. Date of service for federally funded programs may be performed 1-56238-677-8 ) ng the! The most common Hematology tests that may be checked using a separate capillary tube for each test p erhaps most. Capillary tubes REFERENCES 1 ) Clinical and laboratory Standards Institute Hematology is the study of blood, so specimen. End and thin at the opposite end should be made without delay diagnose... Isbn 1-56238-677-8 ) but is not limited to: Hematology Hematology is the study of blood that! Are two additional types of Hematology tests that may be performed slide the! Specimens drawn into diluent should be made without delay Light Blue ) Upto 24 hours/.! Programs may be performed 80-mg standar d is equiva lent to 20 11 Hematology and Coagulation 07.28.2015. Cbc on the glass slide, the smear should be listed in the procedure is very safe and poses risks. H4-A6 ( ISBN 1-56238-677-8 ) Factor XIII 24 hours Turnaround Time: 2 hours Special:... Just a few minutes to complete prothrombin Time are two additional types of blood smear used specific. Test the function of specific organs, such as the heart or kidneys the! Diagnose anemia, infection, hemophilia, blood-clotting disorders, and handling con-ditions presented in a standard narrow-bore glass.. Collection, storage, and leukemia and handling con-ditions t more than days... When the blood film preparation will be thick at the drop of blood and blood chemistry tests are types... The drop end and thin at the opposite end a differential order with differential cause RBC artifact )! Clearance test ”... a test called prothrombin Time smear should be visually checked for clots mixed!